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KMID : 0903519850280040271
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 4 p.271 ~ p.277
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Determination of Respiratory Activity of Mitochondria and Submitochondrial Particles by Using Dropping Mercury Electrode
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Abstract
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A polarograph with specially designed cell compartment usable in kinetic study of the mitochondrial respiration of a small sized sample was made, and its performance and experimental conditions were examined. An applied potential (ca-1.2V vs. SCE) which gives rise to the second step reduction of oxygen caused a considerable level of a residual current independent of oxygen, which is temporarily interpreted as the reduction current of the membrane-bound redox materials) of mitochondria. A potential corresponding to the first step reduction of oxygen (ca-0.4V vs SCE) did not produce the residual current. Thus, it is suggested that a measurement of oxygen concentration in a sample of mitochondria and submitochondrial particles by using dropping mercury electrode should be done kith an applied potential of about -0.4V vs SCE. Consumption of oxygen by mitochondria was observed to follow practically zero order kinetics. Its rate constant exhibited the proportional relationship with the respiratory activity of mitochondria. Usefulness of the instrument was properly demonstrated in the work on the temperature effect on the respiration of mitochondria isolated from several plant tissues which were selected on the basis of chilling susceptivity.
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